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eclipse ti2 fluorescence microscope  (Nikon)


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    Structured Review

    Nikon eclipse ti2 fluorescence microscope
    Eclipse Ti2 Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 12150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti2+fluorescent+microscope/ECLIPSE+Ti2/pm42886891-132-15-14
    Average 99 stars, based on 12150 article reviews
    eclipse ti2 fluorescence microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: As a positive control for G-mtCEPIA2 functioning, 2.5 μM of ionomycin (Alomone Labs) was added at the 4 min. Only cells that displayed proper G-mtCEPIA2 levels were selected. .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 40X1.3 NA Plan Fluor DIC H N2 Oil objective, a pco.edge 4.2bi sCMOS camera and its accompanying software. mCherry and G-mtCEPIA2 were both illuminated through excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm and 470 nm respectively, and the cubical excitation filter set FF01-378/474/554/635 and bandpass emission filters of 595/31 and 515/30, respectively (Semrock, Rochester, USA), for the evaluation of overexpression of the pCMV24 constructs. ..

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 20X0.5 NA Plan Fluor DIC N2 air objective, a pco.edge 4.2bi sCMOS camera and the accompanying software. mCherry was illuminated by means of excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm, a cubical excitation filter set FF01-378/474/554/635 and a bandpass emission filter of 595/31 (Semrock, Rochester, USA) for the evaluation of overexpression of the pCMV24 constructs. .. [Ca2+]cyt measurements were performed each second by alternately exciting Fura-2 at 340 nm (20 ms at 50%) and 380 nm (200 ms at 25%) using a CoolLED pE-300 ultra/pE-340 lamp (CoolLED, Andover, UK), a dichroic mirror FF01-432/515/595/730 and a bandpass emission filter 515/30 (Semrock, Rochester, USA).

    Article Title: In Vitro and In Vivo Evaluation of Decellularized Porcine Femoral Aorta Reinforced With Electrospun Coarse Polycaprolactone Fibers for Vascular Graft Application.
    Article Snippet: .. Fluorescence imaging was visualized using a Nikon Eclipse Ti2 fluorescent microscope (Japan). ..

    Article Title: Effect of Shear-Induced Macrophage Secretome on Endothelial Permeability.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Methods and compositions for delivery of biotin to mitochondria
    Article Snippet: .. Cells were then covered with live cell image buffer and imaged using Nikon Eclipse Ti2 fluorescent microscope (20× objective). .. Cells were then covered with live cell image buffer and imaged using Nikon Eclipse Ti2 fluorescent microscope (20× objective).

    Article Title: Autologous Blood Clot Therapy for Wounds: Investigating the Chemotactic Effect on PBMCs and Fibroblasts in Diabetes.
    Article Snippet: .. The nuclei of cells were stained with NucBlue Live Ready Probes and microscopy performed (Nikon Eclipse TI2 fluorescent microscope, x20 objective). ..

    Article Title: Autologous Blood Clot Therapy for Wounds: Investigating the Chemotactic Effect on PBMCs and Fibroblasts in Diabetes
    Article Snippet: .. The nuclei of cells were stained with NucBlue Live Ready Probes and microscopy performed (Nikon Eclipse TI2 fluorescent microscope, x20 objective). ..

    Software:

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: As a positive control for G-mtCEPIA2 functioning, 2.5 μM of ionomycin (Alomone Labs) was added at the 4 min. Only cells that displayed proper G-mtCEPIA2 levels were selected. .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 40X1.3 NA Plan Fluor DIC H N2 Oil objective, a pco.edge 4.2bi sCMOS camera and its accompanying software. mCherry and G-mtCEPIA2 were both illuminated through excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm and 470 nm respectively, and the cubical excitation filter set FF01-378/474/554/635 and bandpass emission filters of 595/31 and 515/30, respectively (Semrock, Rochester, USA), for the evaluation of overexpression of the pCMV24 constructs. ..

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 20X0.5 NA Plan Fluor DIC N2 air objective, a pco.edge 4.2bi sCMOS camera and the accompanying software. mCherry was illuminated by means of excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm, a cubical excitation filter set FF01-378/474/554/635 and a bandpass emission filter of 595/31 (Semrock, Rochester, USA) for the evaluation of overexpression of the pCMV24 constructs. .. [Ca2+]cyt measurements were performed each second by alternately exciting Fura-2 at 340 nm (20 ms at 50%) and 380 nm (200 ms at 25%) using a CoolLED pE-300 ultra/pE-340 lamp (CoolLED, Andover, UK), a dichroic mirror FF01-432/515/595/730 and a bandpass emission filter 515/30 (Semrock, Rochester, USA).

    Over Expression:

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: As a positive control for G-mtCEPIA2 functioning, 2.5 μM of ionomycin (Alomone Labs) was added at the 4 min. Only cells that displayed proper G-mtCEPIA2 levels were selected. .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 40X1.3 NA Plan Fluor DIC H N2 Oil objective, a pco.edge 4.2bi sCMOS camera and its accompanying software. mCherry and G-mtCEPIA2 were both illuminated through excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm and 470 nm respectively, and the cubical excitation filter set FF01-378/474/554/635 and bandpass emission filters of 595/31 and 515/30, respectively (Semrock, Rochester, USA), for the evaluation of overexpression of the pCMV24 constructs. ..

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 20X0.5 NA Plan Fluor DIC N2 air objective, a pco.edge 4.2bi sCMOS camera and the accompanying software. mCherry was illuminated by means of excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm, a cubical excitation filter set FF01-378/474/554/635 and a bandpass emission filter of 595/31 (Semrock, Rochester, USA) for the evaluation of overexpression of the pCMV24 constructs. .. [Ca2+]cyt measurements were performed each second by alternately exciting Fura-2 at 340 nm (20 ms at 50%) and 380 nm (200 ms at 25%) using a CoolLED pE-300 ultra/pE-340 lamp (CoolLED, Andover, UK), a dichroic mirror FF01-432/515/595/730 and a bandpass emission filter 515/30 (Semrock, Rochester, USA).

    Construct:

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: As a positive control for G-mtCEPIA2 functioning, 2.5 μM of ionomycin (Alomone Labs) was added at the 4 min. Only cells that displayed proper G-mtCEPIA2 levels were selected. .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 40X1.3 NA Plan Fluor DIC H N2 Oil objective, a pco.edge 4.2bi sCMOS camera and its accompanying software. mCherry and G-mtCEPIA2 were both illuminated through excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm and 470 nm respectively, and the cubical excitation filter set FF01-378/474/554/635 and bandpass emission filters of 595/31 and 515/30, respectively (Semrock, Rochester, USA), for the evaluation of overexpression of the pCMV24 constructs. ..

    Article Title: Anti-apoptotic BCL-2 binds to all three IP3R isoforms, thereby limiting the Ca2+-flux properties of IP3R homo-tetramers.
    Article Snippet: .. Next, the four-chamber slide was mounted on a custom slide holder and imaged using a Nikon Eclipse Ti2 fluorescent microscope equipped with a 20X0.5 NA Plan Fluor DIC N2 air objective, a pco.edge 4.2bi sCMOS camera and the accompanying software. mCherry was illuminated by means of excitation using a CoolLed pR-4000 lamp (CoolLED, Andover, UK) at 550 nm, a cubical excitation filter set FF01-378/474/554/635 and a bandpass emission filter of 595/31 (Semrock, Rochester, USA) for the evaluation of overexpression of the pCMV24 constructs. .. [Ca2+]cyt measurements were performed each second by alternately exciting Fura-2 at 340 nm (20 ms at 50%) and 380 nm (200 ms at 25%) using a CoolLED pE-300 ultra/pE-340 lamp (CoolLED, Andover, UK), a dichroic mirror FF01-432/515/595/730 and a bandpass emission filter 515/30 (Semrock, Rochester, USA).

    Fluorescence:

    Article Title: In Vitro and In Vivo Evaluation of Decellularized Porcine Femoral Aorta Reinforced With Electrospun Coarse Polycaprolactone Fibers for Vascular Graft Application.
    Article Snippet: .. Fluorescence imaging was visualized using a Nikon Eclipse Ti2 fluorescent microscope (Japan). ..

    Imaging:

    Article Title: In Vitro and In Vivo Evaluation of Decellularized Porcine Femoral Aorta Reinforced With Electrospun Coarse Polycaprolactone Fibers for Vascular Graft Application.
    Article Snippet: .. Fluorescence imaging was visualized using a Nikon Eclipse Ti2 fluorescent microscope (Japan). ..

    Article Title: Effect of Shear-Induced Macrophage Secretome on Endothelial Permeability.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    other:

    Article Title: Methods and compositions for delivery of biotin to mitochondria
    Article Snippet: HK-2 cells were fixed with ethanol:methanol (1:1) for 30 min at −20° C., permeabilized with 0.1% Triton X-100/PBS for 10 min at RT, blocked with 2% goat serum for 30 min at RT, and incubated with primary mouse anti-PCNA antibody (Dako Agilent, Santa Clara, CA) and secondary goat anti-mouse IgG-BI for 30 min at RT.

    Staining:

    Article Title: Autologous Blood Clot Therapy for Wounds: Investigating the Chemotactic Effect on PBMCs and Fibroblasts in Diabetes.
    Article Snippet: .. The nuclei of cells were stained with NucBlue Live Ready Probes and microscopy performed (Nikon Eclipse TI2 fluorescent microscope, x20 objective). ..

    Article Title: Autologous Blood Clot Therapy for Wounds: Investigating the Chemotactic Effect on PBMCs and Fibroblasts in Diabetes
    Article Snippet: .. The nuclei of cells were stained with NucBlue Live Ready Probes and microscopy performed (Nikon Eclipse TI2 fluorescent microscope, x20 objective). ..



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